Solid feeding and co-culture strategies for an efficient enzymatic hydrolysis and ethanol production from sugarcane bagasse

The feasibility of second-generation (2G) processes still highly depends on improvements in the hydrolysis and fermentation steps, ensuring whole use biomass sugars an integrated approach. main bottlenecks are differences between optimal conditions for both limitations high solid load (HSL) hydrolysis, hexoses pentose co-fermentation. To overcome these problems, present work explored a feeding strategy to improve HSL enzymatic hydrothermally pretreated sugarcane bagasse (PSB), at suboptimal temperatures, further with non-recombinant yeast co-culture (Saccharomyces cerevisiae Kluyveromyces marxianus) sugar entire PSB fraction was used without conditioning. Efficient achieved using helical stirred bioreactor (64 75% cellulose conversion 22.5% solids, 72 h 35 50 °C, respectively), obtaining hydrolysates glucose xylose concentrations (100 g/L). Simultaneous saccharification co-fermentation (SSCF) provided (98%) (52%) 24 deep-well plates, ethanol yield 0.23 getOH/gS, but faced problems cell viability loss bioreactor. Sequential SCF (SqSCF) stood out as promising sugars. results this study provide significant contribution intensification 2G production, addition highlighting difficulties process integration aiming full coming from hydrolysis.